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| Registros recuperados: 240 | |
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| Mroginski,Luis A.; Sansberro,Pedro A.; Scocchi,Adriana M.; Luna,Claudia; Rey,Hebe Y.. |
| Tropical Ilex species have recalcitrant seeds. This work describes experiments demonstrating the feasibility of long-term conservation of Ilex brasiliensis, I. brevicuspis, I. dumosa, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos were aseptically removed from the seeds and precultured (7 days) in the dark, at 27± 2ºC on solidified (0.8% agar) 1/4MS medium, [consisting of quarterstrength salts and vitamins of Murashige and Skoog (1962) medium] with 3% sucrose and 0.1 mg/l Zeatin. The embryos were then encapsulated in 3% calcium alginate beads and pretreated at 24 h intervals in liquid medium supplemented with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Encapsulation-dehydration; Germplasm preservation; Liquid nitrogen; Llex. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100004 |
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| Zou,Yi; Zhong,Wenping. |
| PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Full length PEPP2 associated with both plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microtubule; Membrane; Phosphoinositide; Microviscosity; Wortmannin. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300005 |
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| Pires Bione,Nilton Cesar; Suely Pagliarini,Maria; Alves de Almeida,Leones. |
| A spontaneous male-sterile, female-fertile mutation affecting bivalent arrangement at the metaphase plate and cytokinesis was detected in line BR98-197 of the soybean breeding program developed by Embrapa - National Soybean Research Centre. Untill diakinesis, meiosis was normal with chromosome pairing as bivalents. From this phase, in several meiocytes, bivalents were not able to organize a single metaphase plate and remained scattered in the cytoplasm in a few or several groups. In these meiocytes, chromosomes segregated in both divisions giving rise to several micronuclei. However, the main cause of male sterility was the absence of cytokinesis after telophase II. Instead of the typical tetrads of microspores, four nucleate coenocytic microspores were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Meiosis; Metaphase plate; Cytokinesis; Male sterility. |
| Ano: 2005 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200004 |
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| Capani,Francisco; Saraceno,Ezequiel; Boti,Valeria Romina; Aon-Bertolino,Laura; Fernández,Juan Carlos; Gato,Fernando; Krause,Maria Sol; Giraldez,Lisandro; Ellisman,Mark H.; Coirini,Héctor. |
| Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: F-actin; Phalloidin-eosin phootoxidation; 3-D reconstructions; Electron tomography. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100001 |
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| Solari,Alberto J.; Rahn,Monica I.; Saura,Alicia; Lujan,Hugo D.. |
| The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs in a dorsal-ventral direction, with the resulting nuclear pairs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Giardia; Primitive mitosis; Fine structure of mitosis; Protist mitosis; Cell differentiation. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300004 |
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| Registros recuperados: 240 | |
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